Peptide and protein scaffolds for biotherapeutic discovery and development Research in the Bradley laboratory utilizes approaches at the interface of chemistry, biochemistry, and neurobiology to discover and develop peptide and protein-based molecules as platforms for various medical, biotechnical, and biotherapeutic applications. We are using molecular design and screening techniques to develop molecules with desired biological activities and properties. In addition, biochemical and neurobiological characterization will contribute towards understanding the basis of the native molecule's function.

Fuqua JL, Littrell OM, Lundblad M, Turchan-Cholewo J, Abdelmoti LG, Galperin E, Bradley LH, Cass WA, Gash DM, Gerhardt GA. (2014) Dynamic Changes in the Dopamine Neuron Function after DNSP-11 Treatment: Effects in vivo and Increased ERK 1/2 Phosphorylation in vitro. Peptides 54: 1-8. Hardy PA, Keeley D, Schorn G, Forman, E, Ai Y, Zhang Z, Bradley LH. (2013) Convection enhanced delivery of gadolinium-tagged polylysine. J Neuroscience Methods, 219: 169-175. Littrell OM, Fuqua JF, Richardson AD, Turchan-Cholewo J, Hascup ER, Huettl P, Pomerleau F, Bradley LH, Gash DM, Gerhardt GA. (2013) A synthetic five amino acid GDNF propeptide increases dopamine neuron differentiation and neurochemical function. Neuropeptides, 47: 43-49. Magnani R, Chaffin B, Dick E, Bricken ML, Houtz RL, Bradley LH. (2012) Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins. Protein Expression & Purification, 86: 83-88. Sexton T, Boock LJ, Rodgers DR, Bradley LH, Hersh LB. (2012) Active site mutations change the substrate cleavage specificity of neprilysin. PLoS ONE, 7(2): e32343. Arai R, Kobayashi N, Kimura A, Sato T, Wang AF, Platt JM, Bradley LH, Hecht MH. (2012) Domain-swapped dimeric structure of a de novo 4-helix bundle protein, WA20. J Phys Chem B, 116: 6569-7032. Bradley LH, Bricken ML, Randle C. (2011) Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker. Protein Expression & Purification 75: 186-192. Kelps KA, Turchan-Cholewo J, Hascup ER, Taylor TL, Gash DM, Gerhardt GA, Bradley LH. (2011) Evaluation of the physical and in vitro protective activity of three synthetic peptides derived from the pro- and mature GDNF sequence. Neuropeptides 45(3): 213-218. Bradley LH, Fuqua J, Richardson A, Turchan-Cholewo J, Ai Y, Kelps KA, Glass J, He X, Zhang Z, Grondin R, Littrell OM, Gash DM, Gerhardt GA. (2010) Dopamine neuron stimulating actions of a GDNF propeptide. PLoS ONE 5(3): e9752. Patel SC, Bradley LH, Jinadasa SP, Hecht MH. (2009) Cofactor Binding and Enzymatic Activity in an Unevolved Superfamily of De Novo Designed 4-Helix Bundle Proteins. Protein Science, 18:1388-1400. Bradley LH, Thumfort PP, Hecht MH. (2006) De novo proteins from binary patterned combinatorial libraries. Methods Mol Biol 340: 53-69. Bradley LH, Kleiner RE, Wang AF, Hecht MH, Wood DW. (2005) An Intein-Based Genetic Selection Enables Construction of a High-Quality Library of Binary Patterned De Novo Protein Sequences. Protein Engineering, Design & Selection 18: 201-207.